We report the first evidence of Shimoni bat virus (SHBV) and the detection of Lagos bat virus (LBV)and Mokola virus (MOKV) in bats from Nigeria. In total, 356 bats representing 7 genera and 8 species, collected in 8 locations of northern Nigeria. None of the 356 bat brains screened by direct fluorescent antibody (DFAT) test were positive for <i>lyssavirus</i> antigens. Of the 76 sera screened by modified rapid fluorescent focus inhibition test (RFFIT) 24 (31.6%) had neutralizing activity; 22(29.0%) neutralized LBV, 6(7.9%) neutralized MOKV and 18(23.7%) neutralized SHBV). Lyssaviruses may be circulating in bats or other wild animals in Nigeria.