The Defense Threat Reduction Agency Chemical and Biological Technologies Directorate (DTRA CB) is funding the development of the Biosurveillance Ecosystem (BSVE). Biosurveillance operational capability gaps were analyzed through work process discussions with several disease surveillance organizations. Two meta-workflows were evident. In the first type, epidemiologists identify and characterize health-impacting events to enable community-level responses to the event. The second type is more focused on informing leadership and response in the form of policy modification. Analysts described features of a future biosurveillance environment that they wish were available in various categories: data, enhanced search, verification, analytics, collaboration and communication, and archival.